本发明专利技术公开了用于培养包括李斯特菌（Listeria spp.）的微生物的方法的，包含非螯合镁离子和除氧剂的培养基。所述培养基可以用于培养样品以检测样品中细菌的存在。本发明专利技术还公开了包含用于补充培养基的无镁螯合缓冲液和除氧剂的配方。
【技术实现步骤摘要】
【国外来华专利技术】【专利摘要】本专利技术公开了用于培养包括李斯特菌（Listeria?spp.）的微生物的方法的，包含非螯合镁离子和除氧剂的培养基。所述培养基可以用于培养样品以检测样品中细菌的存在。本专利技术还公开了包含用于补充培养基的无镁螯合缓冲液和除氧剂的配方。【专利说明】用于李斯特菌的培养基和培养方法以及检测李斯特菌的方 法
技术介绍
1.
本专利技术公开的主题涉及用于培养微生物和检测微生物的培养基和方法。2.相关文献美国专利申请N0.10\597909，用于李斯特菌(LISTERIA spp.)的选择性生长培养基，申请日2005年2月12，，公开了包括氯化锂和选择试剂的成分。Mendonca，A.，F.和 Knabel，S.，J.1994.A novel strictly anaerobic recoveryand enrichment system incorporating lithium for detection of heat-1njuredListeria monocytogenes in pasteurized milk containing background microflora.App1.Environ.Microbiol.60:4001-4008.Beumer R.R.，te Giffel M.C.，Anthonie S.V.R.，Cox L J.1996.The effect ofacriflavine and nalidixic acid on the growth of Listeria spp.1n enrichmentmedia.F.Microbiol.13 (2): 137-148.Teoj A., Y-L., Knabel, S., J.2000.Development of a SimpleRecovery-Enrichment System for Enhanced Detection of Heat-1njured Listeriamonocytogenes in Pasteurized Milk.J.Food Prot.63 (4):462-472.Busch SVj DonnelIy CW.Development of a repair-enrichment brothfor resuscitation of heat-1njured Listeria monocytogenes and Listeriainnocua.1992.App1.Environ.Microbiol.58 (I): 14-20.Buchrieser C，Rocourt J.2007.The genus Listeria and Listeriamonocytogenes:phy1genetic position,taxonomy,and identification.1mListeriaj listeriosis, and food safety, 3rd edition./Ed.by Ryser ET，MarthEH.CRC Press.896p.Lang Halter E，Neuhaus K，Scherer S.2012.Listeria weihenstephanensissp.nov., isolated from the water plant Lemna trisulca of a German fresh waterpond.1nt J Syst Evol Microbiol.Apr27..Bertsch D，Rau Jj Eugter MR, Haug MCj Lawson PA, Lacroix C，Mei Ie L 2012.Listeria fleischmannii sp.nov., isolated from cheese.1nt J Syst Evol Microbiol.Apr20..Painter J，Slutsker L.Listeriosis in humans.2007.The genus Listeriaand Listeria monocytogenes:phylogenetic position,taxonomy, and identification.1mListeriaj listeriosis, and food safety, 3rd edition./Ed.by Ryser ET，MarthEH.CRC Press.896p.Wesley IV.Listeriosis in animals.2007.The genus Listeria andListeria monocytogenes:phylogenetic position,taxonomy,and identification.ImListeriaj listeriosis, and food safety, 3rd edition./Ed.by Ryser ET，MarthEH.CRC Press.896p.Ramaswamy V，Cresence VMj Rejitha JSj Lekshmi MUj Dharsana KSj PrasadSPj Vijila HM.2007.Listeria - review of epidemiology and pathogenesis.JMicrobiol Immunol Infect.40(I):4-13.】Koch J，Stark K.Significant increase of listeriosis inGermany—epidemiological patterns2001-2005.(2006).Euro Surveill.11(6):85-8.Anonymous.1ncidence of Foodborne Illness, 2010.(http: //www.cdc.gov/Features/dsFoodbornelllness/).2009欧盟关于人畜共患疾病和人畜共患疾病药剂和食物4专染疫f青白勺趋势和来源白勺摘要?艮告(http://www.efsa.europa.eu/en/efsajournal/doc/2090, pdf).Scallan Ej Hoekstra RMj Angulo FJj Tauxe RVj Widdowson M-Aj Roy SLj JonesJL，Griffin PM.Foodborne illness acquired in the United States - majorpathogens.2011.Emerg Infect Dis.17 (I): 126 - 8.Gandhi M，Chikindas ML 2007.Listeria:A foodborne pathogen that knows howto survive.1nt J Food Microbiol.113(1): 1-15.USDA-FSIS.2011.1solation and Identification of Listeria monocytogenesfrom red meat,本文档来自技高网...
【技术保护点】
培养基，包含具有生物有效浓度的：a)非螯合镁离子；和b)除氧剂。
【技术特征摘要】
【国外来华专利技术】...
【专利技术属性】
技术研发人员：马汀内兹·加布里耶拉，克拉沃·戴维，马杜罗·莱拉，
申请(专利权)人：食品安全测试系统公司，
类型：发明
国别省市：加拿大;CA
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