The invention relates to a method for extracting 37 total saponins by continuous countercurrent ultrasonic extraction combined with membrane technology, belonging to the technical field of extraction and separation of effective components of plants. This method is mainly coarse powder plus 37 weight of 6 to 15 times from 40 to 90% ethanol in 20 ~ 50 DEG C for continuous countercurrent extraction of 20 ~ 120min, ultrasonic frequency is 20 ~ 80KHz; 1000 ~ 2500 of the filtrate with ultrafiltration membrane or 100-800 membrane at 20 to 50 DEG C, 0.4 ~ 4.1MPa film concentration; pressure reducing concentration to no alcohol taste after diluted with water or diluted with water, filtering on macroporous adsorption resin, then washed by 50 ~ 90% ethanol elution; alkaline anion exchange resin with 100-800 nanofiltration membrane, 0.4 ~ 4.1MPa membrane concentration, decolorization and concentrating, spray drying. The method has the advantages of low temperature extraction, high extraction rate, continuous production and high purification rate, and the product quality is ensured and the method is simple and convenient.
【技术实现步骤摘要】
【技术保护点】
１．一种连续逆流超声提取结合膜技术提取三七总皂苷的方法，其特征在于按下述顺序的步骤进行：（１）取三七粉碎成三七粗粉，加入三七粗粉重量６～１５倍的以质量分数计为４０～９０％乙醇在２０～５０℃的温度下进行连续逆流超声提取２０～１２０ｍｉｎ，超声频率为２０～８０ＫＨｚ；（２）乙醇超声提取液过滤，滤液用截留相对分子量为１０００～２５００的超滤膜在２０～５０℃，操作压力０．４～４．１ ＭＰａ下进行膜浓缩，或用截留相对分子量为１００－８００的纳滤膜在２０～５０℃，操作压力０．４～４．１ＭＰａ下进行膜浓缩；（３）膜浓缩液减压浓缩至无醇味后加水稀释至三七粗粉投料重量的４～８倍或直接加水稀释至以质量分数计含乙醇量达１０～２０％，过滤，上于聚苯乙烯型大孔吸附树脂吸附，用水洗脱，水洗液弃去，再用以质量分数计为５０～９０％乙醇洗脱；（４）乙醇洗脱液过碱性阴离子树脂；（５）阴离子树脂透过液用截留相对分子量为１００－８００的纳滤膜，操作压力０．４～４．１ ＭＰａ下进行膜浓缩；（６）膜浓缩液加入为膜浓缩液重量的１～５％的活性炭搅拌脱色；（７）活性炭脱色液过滤后减压浓缩至无醇味后喷雾干燥。
【技术特征摘要】
【专利技术属性】
技术研发人员：武正才，李文，赵锋宁，李明杰，黄春球，
申请(专利权)人：云南植物药业有限公司，
类型：发明
国别省市：53
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