一种卡马西平高效降解菌的提取方法技术

本发明专利技术涉及一种卡马西平高效降解菌的提取方法，包括：（１）取城市污水处理厂二沉池的活性污泥按５ｗｔ％的接入量接种于无机盐培养基中，以梯度压力式驯化法驯化２个月；（２）将驯化２个月后的样品静置３０ｍｉｎ，取上层水样１ｍｌ作梯度稀释，然后每种稀释浓度分别取１ｍｌ菌液于接种到固体培养基进行富集培养，确保长出纯的单一菌株，再接种到斜面培养基，于４℃下保存，即可。该方法从城市污水处理厂二沉池的活性污泥中提取卡马西平降解菌株，种源易得且分离纯化方法简便快捷，成本低，对环境友好；提取到的菌株对卡马西平的降解率可达７５％以上，具有良好的应用前景。

Method for extracting C Masi Bing high-efficiency degrading bacteria

Including the extraction method, the invention relates to a high efficient degradation bacteria C Masi Bing: (1) activated sludge from the city sewage treatment plant two sedimentation tank according to access 5wt% inoculated in inorganic salt medium, the pressure gradient domestication method domestication of 2 months; (2) the domestication of 2 months after the sample the static 30min, take the upper water 1ml gradient dilution, then each dilution were taken 1ml broth medium enriched culture inoculated to ensure a solid, pure single strains, and then inoculated into the slant medium, to 4 DEG C can be saved. The method of extracting C Masi Bing degrading bacteria from activated sludge two sink the pond city sewage treatment plant, which is easy to get and source separation and purification method is simple, low cost, environmentally friendly; extraction to the degradation of C Masi Bing rate is more than 75%, has a good application prospect.

【技术实现步骤摘要】

【技术保护点】
一种卡马西平高效降解菌的提取方法，包括：　　（１）定向驯化培养　　取活性污泥按５ｗｔ％的接入量接种于无机盐培养基中，以梯度压力式驯化法驯化２个月；　　（２）菌株的富集分离和纯化　　将驯化２个月后的样品静置３０ｍｉｎ，取上层水样１ｍＬ作梯度稀释，然后每种稀释浓度分别取１ｍＬ菌液于接种到固体培养基进行富集培养，确保长出纯的单一菌株，再接种到斜面培养基，于４℃下保存，即可。

【技术特征摘要】

【专利技术属性】
技术研发人员：薛罡，杨林，刘亚男，龚清杰，高品，史锦，程起跃，周宁娟，王志楼，
申请(专利权)人：东华大学，
类型：发明
国别省市：31[]