本发明专利技术提供一种抗干扰能力强、稳定性好的血清尿素检测方法及试剂。采用酶偶联速率法，可以有效检测血清尿素的含量，具有抗干扰能力强、稳定性好等优点。该产品包括试剂R1和试剂R2，采用新的缓冲体系和稳定剂，显著改善了试剂的稳定性。同时采用新型表面活性剂烷基糖苷，不仅显著改善测定的性能，而且增强了试剂的稳定性和抗干扰能力，完全可以满足临床需要。
【技术实现步骤摘要】
本专利技术设及用于临床测定血清中尿素含量的检测方法及试剂，属于临床体外检测
技术介绍
尿素是蛋白质分解代谢的含氮终产物。尿素氮大约占血液中非蛋白氮的75%，它通 过肝脏中的氨进行合成，是蛋白质脱氨作用的产物。通过肾小球从血液中过滤尿素到尿中， 是消除体内多余氮的主要方法。尿素氮的检测用于诊断和治疗某些肾脏疾病和代谢素乱。 血液尿素水平是肾功能W及肾前状态和肾后状态的度量标准，肾前因素引起的尿 素的升高包括屯、脏代偿失调，缺水或增加的蛋白质分解代谢。水平增加的肾脏因素有急性 肾小球肾炎，慢性肾炎，多囊肾，肾纤维化和肾小管坏死。 目前临床实验室测定尿素最常用的方法有二己酷-目亏法，脈酶-波氏比色法，酶偶 联速率法。 二己酷一目亏法；尿素可与二己酷作用，在强酸加热的条件下，生成粉红色的二嗦化 合物（Fearom反应)，在540皿比色，其颜色强度与尿素含量成正比。二己酷不稳定，用二己 酷一目亏代替，后者遇酸水解成二己酷。此外，二己酷一目亏与尿素的反应不是专一的，与瓜氨 酸也有显色。本法专一性差，线性范围狭窄，且试剂中含有强腐蚀性化学物，易对仪器和环 境造成污染，一般临床已很少使用此方法。 脈酶一波氏法：首先用脈酶水解尿素生成氨和二氧化碳。然后，氨在碱性介质中与 苯酪及次氯酸反应，生成藍色的嘲噪酪，此过程需用亚硝基氯化钢催化反应。藍色嘲噪酪的 生成量与尿素含量成正比，在630皿波长比色测定。该法的缺点在于易受环境中锭根离子 的影响造成结果假性偏高，此外脈酶的活性容易受到氣化物的抑制造成结果偏低。
技术实现思路
本专利技术提供一种抗干扰能力强、稳定性好的血清尿素检测方法及试剂，该产品包 括试剂R1和试剂R2。试剂R1;【主权项】1. 一种稳定、抗干扰能力强的血清尿素检测方法及试剂，其特征在于包括试剂Rl和试 剂R2,所述试剂Rl和试剂R2的组成如下： 试剂Rl : PIPES (哌嗪-1，4-二乙磺酸）缓冲液（pH=7. 6)...................................IOOm mol/L a _酮戊Z酸.................................................................................................20m mol/L 谷氨酸脱氢酶............................................................................................80 0U/L ADP...........................................................................................................2m mol/L BSA............................................................................................................ lg/L 賴IS......................................................................................................... 2g/L mm............................................................................................................2 Og/L APG (烷基糖苷）........................................................................................... 2g/L 魏，内（NaN3)...........................................................................................0. 5g/L 试剂R2 : PIPES (哌嗪-1，4-二乙磺酸）缓冲液（pH=9. 4)...................................100m mol/L 臟...........................................................................................................10 KU/L NADH........................................................................................................I. 2m mol/L BSA............................................................................................................ lg/L 賴IS......................................................................................................... 2g/L mm............................................................................................................2 Og/L APG (烷基糖苷）.......................................................................................... 2g/L 魏，内（NaN3)...........................................................................................0. 5g/L 〇2. 根据权利要求1所述的尿素检测试剂，其特征在于试剂Rl中缓冲液为25°C，pH为 7. 6的哌嗪-1，4-二乙磺酸缓冲液。3. 根据权利要求1所述的的尿素检测试剂，其特征在于试剂R2中缓冲液为25°C，pH为 9. 4的哌嗪-1，4-二乙磺酸缓冲液。4. 根据权利要求1所述的尿素检测试剂，其特征在于所述稳定剂为BSA、海藻糖、蔗糖。5. 根据权利要求1所述的尿素检测试剂，其特征在于所述表面活性剂为APG (烷基糖 苷）。6. 根据权利要求1所述的尿素检测试剂，其特征在于所述防腐剂为NaN 3。7. -种使用权利要求1-6中任一项所述的尿素检测试剂来检测尿素（UREA)的检测方 法，其特征在于使用本文档来自技高网...
【技术保护点】
一种稳定、抗干扰能力强的血清尿素检测方法及试剂，其特征在于包括试剂R1和试剂R2，所述试剂R1和试剂R2的组成如下：试剂R1：PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液（pH=7.6）···································100mmo1/Lα‑酮戊二酸·································································································20mmo1/L谷氨酸脱氢酶····························································································800U/LADP···········································································································2mmo1/LBSA············································································································1g/L海藻糖·········································································································2g/L蔗糖············································································································20g/LAPG(烷基糖苷)···························································································2g/L叠氮钠（NaN3）···························································································0.5g/L试剂R2：PIPES（哌嗪‑1,4‑二乙磺酸）缓冲液（pH=9.4）···································100mmo1/L脲酶···········································································································10KU/LNADH········································································································1.2mmo1/LBSA············································································································1g/L海藻糖·········································································································2g/L蔗糖············································································································20g/LAPG(烷基糖苷)··························································································2g/L叠氮钠（NaN3）···························································································0.5g/L 。...
【技术特征摘要】
【专利技术属性】
技术研发人员：甘宜梧，李志明，谭柏清，
申请(专利权)人：山东博科生物产业有限公司，
类型：发明
国别省市：山东;37
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